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Chiral application: Pindolol
Separation of pindolol on Kromasil CelluCoat RP. Technology: reversed phase. Present substances: pindolol.
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How can column lifetime be maximized?
More Questions and AnswersColumn plugging is responsible for well over half of all column failures. Therefore thorough filtration (0.2 μm) of mobile phases and samples is a key for long column lifetime.
The mobile phase compatibility is another important factor. Standard RP stationary phases are stable under pH 1.5 to 9. Under certain conditions (organic buffers) the stationary phases are even chemically stable up to pH 12. Please consult with our technical support if you would like to use your classic Kromasil column at pH values exceeding pH 9. Generally, organic buffers are milder towards the silica based stationary phase than inorganic ones, such as phosphates or carbonates. If you need to run your method at high pH choose Kromasil Eternity which is stable up to pH 12.
Further, the columns should never be left in a buffered mobile phase when the flow is interrupted for more than 10 minutes. Either choose a very low flow rate (0.1 ml/min for a 4.6 mm ID column) instead of stopping the flow completely, or exchange any buffered mobile phase with non-buffered one (e.g. water/acetonitrile 70/30). This will prevent the column from being clogged with precipitated buffer salts.
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HPLC media
Kromasil is one of the world’s leading brands for spherical silica for use at any scale of high performance liquid chromatography (HPLC): from the smallest laboratory analytical analysis to the full-scale multi-ton production line, with the same efficiency all the way.
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A column is not merely a vessel for HPLC-media, it is an integrated part of the final product, and it cannot be packed by just anyone. Therefore, all Kromasil Original Columns are packed by us, here at Kromasil, to guarantee the best quality and usage of Kromasil.
Chiral stationary phases
Enantiomer separation is a challenging task to achieve. Kromasil Chiral media are available with four different chemistries that offer a broad range of selectivity for your chiral separation from analytical columns to full process scale bulk media needs.
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Analysis
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You need to quickly purify substances for further studies in the development of pharmaceuticals. Kromasil will help you achieve your goals with ease and efficiency.
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You are using preparative chromatography for high efficiency purification in a pilot, or up to industrial-scale production.
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