Determination of lipophilic antioxidants in plasma. Technology: reversed phase. Present substances: H 290/51, H 290/39.View application
A guard column can be useful if the injection sample consists of a complex matrix with partially unknown content. However, a guard column does never make sample filtration redundant! If a guard column is to provide adequate protection for the analytical column, it must be changed often enough in order to avoid the column performance from deteriorating. By monitoring plate number (N), pressure, and resolution (Rs), the performance of the guard column, as well as the analytical column, can be assessed and a suitable moment for exchanging the guard column can be chosen.
As a rule of thumb, the guard column should be replaced when one of the following points applies:
Kromasil is one of the world’s leading brands for spherical silica for use at any scale of high performance liquid chromatography (HPLC): from the smallest laboratory analytical analysis to the full-scale multi-ton production line, with the same efficiency all the way.
A column is not merely a vessel for HPLC-media, it is an integrated part of the final product, and it cannot be packed by just anyone. Therefore, all Kromasil Original Columns are packed by us, here at Kromasil, to guarantee the best quality and usage of Kromasil.
Enantiomer separation is a challenging task to achieve. Kromasil Chiral media are available with four different chemistries that offer a broad range of selectivity for your chiral separation from analytical columns to full process scale bulk media needs.
You are working with HPLC for the separation and identification of analytical substances in laboratories. Challenge the selectivity of Kromasil for your most sensitive analysis.analysis entry
You need to quickly purify substances for further studies in the development of pharmaceuticals. Kromasil will help you achieve your goals with ease and efficiency.development entry
You are using preparative chromatography for high efficiency purification in a pilot, or up to industrial-scale production.
Kromasil is available in ton quantities to meet your process demands.production entry