• analysis

    You are working with HPLC for the separation and identification of analytical substances in laboratories. Challenge the selectivity of Kromasil for your most sensitive analysis.

  • development

    You need to quickly purify substances for further studies in the development of pharmaceuticals. Kromasil will help you achieve your goals with ease and efficiency.

  • production

    You are using preparative chromatography for high efficiency purification in a pilot or up to industrial-scale production. The robustness of Kromasil is available in ton quantities to meet your process demands.

Application Guide

Sugars

Analysis of sugars

chromatogram

Conditions

Column:Kromasil 100 Å, 5 μm, NH2, 4.6 × 250 mm
Eluent:ACN/water (75/25)
Flow rate:1 ml/min
Detection:Refractive index

Peaks legend

  • 1 = L-rhamnose
    s00395
  • 2 = xylose
    s00401
  • 3 = fructose
    s00402
  • 4 = mannose
    s00403
  • 5 = glucose
    s00397
  • 6 = galactose
    s00404
  • 7 = sucrose
    s00405
  • 8 = maltose
    s00406
  • 9 = lactose
    s00407
  •  

Reference

Eka apllication note

Kromasil product availability

Availability of Kromasil 100 Å, NH2 in columns
Column sizes, mm:
Particle
size, μm		2.1
×
50		2.1
×
100		2.1
×
150		2.1
×
250		3.0
×
50		3.0
×
100		3.0
×
150		3.0
×
250		4.0
×
50		4.0
×
100		4.0
×
125		4.0
×
150		4.0
×
250		4.6
×
50		4.6
×
100		4.6
×
125		4.6
×
150		4.6
×
200		4.6
×
250		10
×
250		21.2
×
150		21.2
×
250		30
×
250		50
×
250
3.5 table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker
5 table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker
7 table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker
10 table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker
13 table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker
16 table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker table marker
AkzoNobel, Separation Products · SE–445 80 Bohus, Sweden · Phone +46 31 58 70 00 · Fax +46 31 58 77 27 · E-mail: kromasil@akzonobel.com