• analysis

    You are working with HPLC for the separation and identification of analytical substances in laboratories. Challenge the selectivity of Kromasil for your most sensitive analysis.

  • development

    You need to quickly purify substances for further studies in the development of pharmaceuticals. Kromasil will help you achieve your goals with ease and efficiency.

  • production

    You are using preparative chromatography for high efficiency purification in a pilot or up to industrial-scale production. The robustness of Kromasil is available in ton quantities to meet your process demands.

Application Guide

ATP degradation products

Determination of ATP degradation products from fish decomposition

chromatogram

Conditions

Column:Kromasil 100 Å, 5 μm, C18, 4.6 × 250 mm
Eluent A:ACN
Eluent B:phosphate buffer (pH 7.00, 60 mM K2HPO"_4 + 40 mM KH2PO4)
Gradient:0 min 100% B, 4 min 98% B, 5 min 97% B, 8 min 96% B, 15 min 96% B, 15.01 min 100% B
Flow rate:1 ml/min
Temperature:25°C
Detection:UV 254 nm

Peaks legend

  • 1 = inosine monophosphate
    s00436
  • 2 = adenosine triphosphate
    s00437
  • 3 = adenosine diphosphate
    s00438
  • 4 = adenosine monophosphate
    s00439
  • 5 = hypoxanthine
    s00045
  • 6 = purine
    s00440
  • 7 = inosine
    s00048
  •  

Reference

M. Vallé, P. Malle, S. Bouquelet (1998) Evaluation of fish decomposition by liquid chromatographic assay of ATP degradation products, J. AOAC Int. 81 (3), 571-575

Kromasil product availability

Availability of Kromasil 100 Å, C18 in columns
Column sizes, mm:
Particle
size, μm		2.1
×
50		2.1
×
75		2.1
×
100		2.1
×
150		2.1
×
250		3.0
×
50		3.0
×
75		3.0
×
100		3.0
×
125		3.0
×
150		3.0
×
250		4.0
×
50		4.0
×
100		4.0
×
125		4.0
×
150		4.0
×
250		4.6
×
50		4.6
×
75		4.6
×
100		4.6
×
125		4.6
×
150		4.6
×
200		4.6
×
250		10
×
250		21.2
×
150		21.2
×
250		30
×
250		50
×
250
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AkzoNobel, Separation Products · SE–445 80 Bohus, Sweden · Phone +46 31 58 70 00 · Fax +46 31 58 77 27 · E-mail: kromasil@akzonobel.com