Equilibration times are time-consuming activities when running chiral chromatography. Higher flow rates allow Kromasil CelluCoat to show substantially shorter equilibration times than other coated phases on the market.
Fig 1: An example of investigated equilibration times with Kromasil CelluCoat.
Common conditions:
Stationary phase: Kromasil CelluCoat, 3 μm
Column size: 4.6 × 150 mm
Mobile phase 1: Heptane/2-Propanol (90/10)
Mobile phase 2: Ethanol
Solute: trans-Stilbene oxide
Flow rate: 1 ml/min
Temperature: 25 °C
Detection: UV 229 nm