Kromasil CelluCoat

CelluCoat

analysis

You are working with HPLC for the separation and identification of analytical substances in laboratories. Challenge the selectivity of Kromasil for your most sensitive analysis.

development

You need to quickly purify substances for further studies in the development of pharmaceuticals. Kromasil will help you achieve your goals with ease and efficiency.

You are using preparative chromatography for high efficiency purification in a pilot or up to industrial-scale production. The robustness of Kromasil is available in ton quantities to meet your process demands.

Loadability of Kromasil CelluCoat

Kromasil products are well known for their ability to work along the whole spectrum from analytical to industrial scale chromatography.

“Works all
the way”

Kromasil CelluCoat is produced in particle sizes of 3, 5 and 10 µm. Since the manufacturing technology is identical for all particle sizes, any development study made on the smaller particles can be translated to a larger column with 10 µm particles.

Examples:

Binaphthol

Analytical injection of Binaphtol on CelluCoat

Preparative injection of Binaphtol on CelluCoat

Analytical injection
Eluent: heptane/2-propanol (90/10)
Sample size: 10 µg
Detection: UV 223 nm

Preparative injection
Eluent: heptane/2-propanol (90/10)
Sample size: 1 mg
Detection: UV 350 nm

	Enantiomer 1	Enantiomer 2
Purity [%]	95.4	97.8
Yield [%]	91.7	72.8

Common conditions:
Phase: Kromasil CelluCoat, 10 µm
Flow rate: 1 ml/min
Column size: 4.6×250 mm
Temperature: 25 °C

Equivalent loading on a
50×250 mm column:
120 mg

Metoprolol

Preparative injection of metoprolol on CelluCoat

Analytical injection
Eluent:
heptane/2-propanol/DEA (90/10/0.1)
Sample size: 10 µg
Detection: UV 223 nm

Preparative injection
Eluent:
heptane/2-propanol/DEA (90/10/0.1)
Sample size: 20 mg
Detection: UV 290 nm

	Enantiomer 1	Enantiomer 2
Purity [%]	100	99.7
Yield [%]	97.9	88.1

Common conditions:
Phase: Kromasil CelluCoat, 10 µm
Flow rate: 1 ml/min
Column size: 4.6×250 mm
Temperature: 25 °C

Equivalent loading on a
50×250 mm column:
2.4 g

· Eka Chemicals, Separation Products · SE–445 80 Bohus, Sweden · Phone +46 31 58 70 00 · Fax +46 31 58 77 27 ·
· E-mail kromasil@eka.com ·