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Kromasil CelluCoat

analysis

You are working with HPLC for the separation and identification of analytical substances in laboratories. Challenge the selectivity of Kromasil for your most sensitive analysis.

development

You need to quickly purify substances for further studies in the development of pharmaceuticals. Kromasil will help you achieve your goals with ease and efficiency.

production

You are using preparative chromatography for high efficiency purification in a pilot or up to industrial-scale production. The robustness of Kromasil is available in ton quantities to meet your process demands.

Negligible memory effects

Additives, like TFA or DEA, are commonly used in chiral chromatography for enhancing the separation of acidic and basic racemates. These additives can strongly interact with the stationary phase in such a way that so-called memory effects occurs. Tedious washing and equilibration may be needed to restore the performance of the phase. In figure 2 is shown a sequence of separations on Kromasil CelluCoat using TFA and DEA as respective additive. The equilibration time in between is relatively short and the performance is retrieved without hassle.

cellucoat_stability_fig02

Fig. 2 - Negligible memory effect of additives on Kromasil CelluCoat.
Conditions:
Stationary phase: Kromasil CelluCoat, 3 μm
Mobile phase 1:
Heptane/2-Propanol/TFA (90/10/0.1)
Mobile phase 2:
Heptane/2-Propanol/DEA (90/10/0.1)
Column size: 4.6 x 150 mm
Flow rate: 1 ml/min
Temperature: 25 °C

· Eka Chemicals, Separation Products · SE–445 80 Bohus, Sweden · Phone +46 31 58 70 00 · Fax +46 31 58 77 27 ·
· E-mail kromasil@eka.com ·