Switching between compatible organic mobile phases will not lead to any degradation in performance
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You can run Kromasil CelluCoat with maintained stability and performance when switching between compatible normal or polar organic phases. Figure 1 illustrates the impact of a mobile phase switch from heptane/2-propanol (90/10) to 100% acetonitrile and back, then to 100% 2-propanol and back. Performance is measured with trans-stilbene oxide as the efficiency on the first eluted peak (Knox plot) and the selectivity. Neither seems hardly affected by the mobile phase switches.
Fig. 1 - Stability of Kromasil CelluCoat. Efficiency (Knox plot) and selectivity.
Conditions:
Solute: trans-Stilbene oxide
Stationary phase: Kromasil CelluCoat, 5 μm
Mobile phase: heptane/2-propanol (90/10)
Column size: 4.6 x 250 mm
Flow rates: 0.1-1.2 ml/min
Temperature: 25 °C