Technical support

• Packing instruction for Kromasil in DAC columns
• Care and use of columns
• Guidelines for chiral chromatography using DMB and TBB
• Optimizing your large-scale application with the software KromaGuide
• Unpacking and care of used packings
• Frequently Asked Questions

Guidelines for kromasil chiral phases

Conditions that are normally applied:

The phases normally give the best separation factors in straight phase mode due to hydrogen bonding, but they are also stable under reversed phase conditions.

The straight phase conditions are normally hexane- or heptane-based with modifiers like:

• tert. butylmethylether
• ethyl acetate
• tetrahydrofuran
• dichloromethane
• isopropanol

• Adjust the modifier content to achieve a k’-value of approx. 1-5
• If your racemate is acidic, add 0.1 - 0.2 % acetic acid
• If your racemate is basic, add 0.1 - 0.2 % acetic acid or a combination of acetic acid (0.1 - 0.2 %) and triethylamine (0.1 %) with an excess of the acid relative to the amine

For the separation of acidic racemates we recommend starting with the TBB phase.