Kromasil Diol
The perfect complementary phase for your NP and RP separations
Kromasil Diol is developed in order to provide excellent performance in HPLC as well as in SFC. By utilizing a high concentration of the dihydroxypropyl functional group, Kromasil Diol shows unique characteristics and provides an alternative selectivity to Kromasil Silica, Cyano, and Amino phases.
Kromasil Diol – the alternative
Many Diol phases on the market show only minor difference in selectivity compared to equivalent bare silica. Due to very high concentration of the dihydroxypropyl functional group our Kromasil Diol exhibits substantial difference in selectivity compared to Kromasil Silica, Kromasil Cyano, and Kromasil Amino. Therefore we propose Kromasil Diol as the perfect complementary phase when searching for highest selectivity!
Conditions:
Stationary phases (respectively): Kromasil 60-5-Diol, Kromasil 60-5-SIL, Kromasil 60-5-CN
Sample composition: 1: tri-tert-butylbenzene, 2: 2-ethoxyaniline, 3: aniline, 4: catechol, 5:
2,4-dinitroaniline, 6: hydroquinone, 7: 4-nitroaniline
Column size: 4.6 x 250 mm
Mobile phase: heptane/2-propanol (85/15)
Flow rate: 2 ml/min, Temperature: 20 °C, Detection: UV 224 nm
Kromasil Diol is based on the unique high performing and high purity Kromasil 60 Å Silica. It is available as bulk and slurry packed columns according to the availability table at the bottom of this page. Other particle sizes and pore sizes are available upon request!
Kromasil Diol – a comparison study
We have injected a mix of phenols on a Kromasil Diol 5 μm analytical column, and compared the result with two commercially available Diol 5 μm analytical columns. In this comparison study we have only included phases showing a pronounced Diol character, and not Diol phases having a character more similar to bare silica.
Common Conditions:
Column size: 4.6 × 250 mm
Sample: tricyclic antidepressants
Elution order: 2-nitrophenol, phenol, 4-nitrophenol, 2-aminophenol, 3-aminophenol
Mobile phase: heptane/2-propanol (75/25)
Flow rate: 2 ml/min
Temperature: 20 °C
Detection: UV 254 nm
Kromasil Diol under RP conditions
Kromasil Diol also provides an excellent selectivity under certain RP conditions. In this particular case, Kromasil Diol nicely separates a mix of polar compounds. Kromasil Diol is wettable, which means that it is working excellent using 100% aqueous conditions.
Conditions:
Sample (elution order): caffeine, theobromine, thymine, uracil, adenine & guanine
Stationary phase: Kromasil 60-5-Diol
Column size: 4.6 × 250 mm
Mobile phase: acetonitrile/ 25 mM potassium phosphate, pH 7.85 (80/20)
Flow rate: 1 ml/min.
Temperature: 20 °C
Detection: UV 270 nm
Kromasil product availability
Available from our distributors and the online shop
Available from our distributors
· E-mail kromasil@akzonobel.com ·