Kromasil Phenyl
For wettability and
alternative selectivity
Kromasil Phenyl is developed to be the perfect alternative to present Kromasil RP phases. The phase is completely wettable, and compatible with 100% aqueous mobile phases. Kromasil Phenyl exhibits a unique selectivity for aromatic compounds, due to a possibility for π-π interactions between the phenyl bonded phase and the solute.
Low silanol activity
Kromasil Phenyl is derivatized using a mono-functional silane, followed by an extensive end-capping. The result is a stationary phase with high stability, high reproducibility, and symmetrical peaks for basic compounds.
A study of Kromasil Phenyl compared to “standard” phenyl phases was performed using a mix of antidepressants. The anti-depressant test at pH 6 is a perfect indicator for silanol activity. The symmetrical peaks for Kromasil Phenyl under these conditions illustrate the very low silanol activity for this phase.
Common Conditions:
Column size: 4.6 × 250 mm
Sample: tricyclic antidepressants
Elution order: uracil (void marker), toluene (hydrophobic marker), nortriptyline, imipramine
& amitriptyline
Mobile phase: methanol/25 mM potassium phosphate pH 6 (80/20)
Flow rate: 1ml/min
Temperature: 20 °C
Detection: UV 215 nm
High chemical stability
The chemical stability of the bonded phase is one of the most important factors determining the lifetime of the column or packing material. A high stability will also minimize leakage from the column.
To investigate the chemical stability, Kromasil Phenyl was flushed continuously for 6 days, using a mobile phase of pH 9, and monitored during and after the test.
Common Conditions:
Stationary phase: Kromasil 100-5-Phenyl
Column size: 4.6 × 250 mm
Conditions for
a Flushing for 6 days with regular toluene tests
Mobile phase: Methanol/Amonium acetate, 10 mM, pH 9 (80/20)
Flow rate during flush: 0.05 ml/min
Flow rate during toluene test: 1 ml/min
Detection: UV 254 nm
Conditions for
b Final antidepressant test
Elution order: uracil (void marker), toluene (hydrophobic marker), nortriptyline, imipramine
& amitriptyline
Mobile phase: methanol/25 mM potassium phosphate pH 6 (80/20)
Flow rate: 1ml/min
Temperature: 20 °C
Detection: UV 215 nm
Enhanced selectivity
Phenyl phases are known to have an enhanced selectivity compared to standard alkyl phases (C4, C8 and C18) for aromatics, due to π-π interactions. When optimizing separations Kromasil Phenyl is therefore an important complementary phase to the regular Kromasil RP phases. This advantage is shown for a mixture of some water-soluble vitamins.
Conditions:
Column size: 4.6 × 250 mm
Mobile phase: acetonitrile/ammonium phosphate, 20 mM (12/88)
Flow rate: 1 ml/min
Temperature: 20 °C
Detection: UV 254 nm
100% aqueous conditions
Kromasil Phenyl is completely compatible with 100% aqueous mobile phases, making it possible to retain polar compounds under these conditions. And in preparative chromatography, when large volumes are injected directly from a previous 100% aqueous chromatographic step, this property of Kromasil Phenyl will prove to be a great advantage. Due to the unique selectivity Kromasil Phenyl is a perfect choice for purification and analysis of aromatic-containing peptides.
Conditions:
Solutes: cysteine, uracil and guanine (in elution order)
Stationary phase: Kromasil 100-5-Phenyl
Column size: 4.6 × 250 mm
Mobile phase: 25 mM potassium phosphate pH 7.85 (100% aq)
Flow rate: 1 ml/min
Temperature: 20 °C
Detection: UV 254 nm
Conditions:
Solutes: felypressin and triptorelin (in elution order)
Stationary phase: Kromasil 100-5-Phenyl
Column size: 4.6 × 250 mm
Mobile phase: acetonitrile/10 mM ammonium acetate pH 4.9
Gradient: 0 – 3 min. 100% buffer, 3.1 – 23.1 min. 15% – 35% acetonitrile, 23.2 – 30 min. 50% acetonitrile
Flow rate: 1 ml/min
Temperature: 20 °C
Detection: UV 254 nm
Kromasil product availability
Available from our distributors and the online shop
Available from our distributors
· E-mail kromasil@akzonobel.com ·