Kromasil Chiral technical evidence

analysis

You are working with HPLC for the separation and identification of analytical substances in laboratories. Challenge the selectivity of Kromasil for your most sensitive analysis.

development

You need to quickly purify substances for further studies in the development of pharmaceuticals. Kromasil will help you achieve your goals with ease and efficiency.

You are using preparative chromatography for high efficiency purification in a pilot or up to industrial-scale production. The robustness of Kromasil is available in ton quantities to meet your process demands.

Loadability of Kromasil Chiral bonded phases

The high loading capacity of Kromasil Chiral is given by the high available surface area of the Kromasil silica and the high chiral ligand density. The loading capacity in preparative scale is of course determined also by the selectivity, α.

Two preparative applications are illustrated in figures 1 and 2 with selectivities of 2.09 and 1.65, respectively.

Analytical and preparative separation of binaphtol

Fig. 1 - Analytical and preparative separation of binaphthol
Conditions, analytical:
Stationary phase: Kromasil Chiral 5 μm DMB
Column size: 4.6 × 250 mm
Flow rate: 1.0 ml/min
Mobile phase: Hexane/MTBE (70/30)
Conditions, preparative:
Stationary phase: Kromasil Chiral 10 μm DMB.
Column size: 50 × 250 mm, DAC
Flow rate: 118 ml/min.
Mobile phase: Hexane/MTBE (70/30)
Load: 1 770 mg.

Fig. 2 - Analytical and preparative separation of naproxen.
Conditions:
Stationary phase: Kromasil Chiral TBB.
Column size: 10 × 250 mm
Flow rate: 4.7 ml/min
Mobile phase: Hexane/Acetone/Acetic acid (92/8/1)

· Eka Chemicals, Separation Products · SE–445 80 Bohus, Sweden · Phone +46 31 58 70 00 · Fax +46 31 58 77 27 ·
· E-mail kromasil@akzonobel.com ·