Kromasil Chiral technical evidence

• Bonded phases - Introduction
• Bonded phases - Mechanical and chemical stability
• Bonded phases - Loadability
• Coated phases - Introduction
• Coated phases - Mechanical stability
• Coated phases - Performance stability
• Coated phases - Loadability

Introduction to Kromasil Chiral coated phases

“Kromasil ^® CelluCoat™ stretches the limits for analytical and industrial chiral chromatography.”

Separation Products has developed Kromasil CelluCoat to meet an increasing demand of stationary phases with a wide chiral discrimination ability and as a complement of the Kromasil Chiral phases DMB and TBB.

The silica

The matrix is a super wide pore silica specially designed to provide the best performance for chiral chromatography applications. Compared to other Kromasil products, this silica has a low surface area. This reduces the number of achiral interaction sites, and thus, increases the chiral selectivity. Also, the silica is available in small particle sizes, down to 3 µm of diameter, which gives high efficiency and high resolution.

Fig. 1 - Electron microsopy - Super wide pore silica particles, close view and trough-cut

The pore size distribution is optimized for the best mechanical strength. Consequently CelluCoat can withstand pressures up to 400 bar, which gives a free working range in most  commercial HPLC systems. This allows high-speed analysis with reduced cycle time compared to traditional adsorbed polysaccharide phases.

The chiral selector

The super wide pore silica is modified with tris-(3,5-dimethylphenyl)carbamoyl cellulose (figure 2). This selector is well-known for it's wide range in chiral chromatography. The selector is applied on the silica using a specially designed coating technology, which ensures homogenous distribution of the selector.

Fig. 2 - The CelluCoat chiral selector