Chiral technical evidence

• Bonded phases - Introduction
• Bonded phases - Mechanical and chemical stability
• Bonded phases - Loadability
• Coated phases - Introduction
• Coated phases - Mechanical stability
• Coated phases - Performance stability
• Coated phases - Loadability

Loadability of Kromasil CelluCoat

“Works all
the way”

Kromasil CelluCoat is produced in particle sizes of 3, 5 and 10 µm. Since the manufacturing technology is identical for all particle sizes, any development study made on the smaller particles can be translated to a larger column with 10 µm particles.

Examples:

• Binaphthol (lower selectivity: 1.16)
• Metoprolol (higher selectivity 2.36)

Binaphthol

Analytical injection Eluent: heptane/2-propanol (90/10) Sample size: 10 µg Detection: UV 223 nm		Preparative injection Eluent: heptane/2-propanol (90/10)   Sample size: 1 mg Detection: UV 350 nm
		Enantiomer 1 Enantiomer 2 Purity [%] 95.4 97.8 Yield [%] 91.7 72.8
Common conditions: Phase: Kromasil CelluCoat, 10 µm Flow rate: 1 ml/min Column size: 4.6×250 mm Temperature: 25 °C		Equivalent loading on a 50×250 mm column: 120 mg

Metoprolol

Analytical injection Eluent: heptane/2-propanol/DEA (90/10/0.1) Sample size: 10 µg Detection: UV 223 nm		Preparative injection Eluent: heptane/2-propanol/DEA (90/10/0.1) Sample size: 20 mg Detection: UV 290 nm
		Enantiomer 1 Enantiomer 2 Purity [%] 100 99.7 Yield [%] 97.9 88.1
Common conditions: Phase: Kromasil CelluCoat, 10 µm Flow rate: 1 ml/min Column size: 4.6×250 mm Temperature: 25 °C		Equivalent loading on a 50×250 mm column: 2.4 g