Kromasil Chiral technical evidence

• Bonded phases - Introduction
• Bonded phases - Mechanical and chemical stability
• Bonded phases - Loadability
• Coated phases - Introduction
• Coated phases - Mechanical stability
• Coated phases - Performance stability
• Coated phases - Loadability

Mechanical stability of Kromasil Chiral coated phases

Packing the material

Kromasil products have a well-known reputation for their high mechanical stability. The silica matrix of CelluCoat is here to maintain this reputation in the world of polysaccharide-bonded chiral phases. The mechanical stability is primordial when it's comes to packing and repacking preparative HPLC-columns. The higher mechanical stability, the longer life-time of the packing material, and thus, the better process economy.

High speed chromatography

The specially designed coating method that is used for Kromasil CelluCoat is also a factor that enhances the mechanical stability of the packing material. Kromasil CelluCoat withstands flow rates equivalent to pressures drop up to 400 bar over the bed. This is otherwise a well-known restriction factor for coated polysaccharide phases. This makes it possible to run analytical chromatography a lot faster within the boundaries of most commercial HPLC systems.

Fig. 2 - Stability under high speed chromatography.
Conditions:
Solute: trans-Stilbene oxide
Stationary phase: Kromasil CelluCoat, 3 µm
Column size: 4.6×50 mm
Mobile phase: Heptane/2-Propanol (90/10)
Flow rate: 7 ml/min
Temperature: 25 °C
Detection: UV 229 nm