Kromasil technical evidence

• Introduction
• Mechanical stability
• Chemical stability
• Surface properties
• Loadability
• Scalability

Introduction

Kromasil silica packings are designed to meet the highest demand in HPLC, SFC and SMB from analytical to process scale.

The uniqueness of Kromasil high performance spherical silica is the combination of:

• high surface area
• mechanical strength

Other outstanding properties are:

• chemical purity
• chemical stability
• optimized surface properties
• well-defined pore structure

Kromasil HPLC silica consists of perfectly spherical, totally porous particles. They are produced in sizes of 3.5, 5, 7, 10, 13 and 16 µm (larger particles can be produced upon request) and with a narrow particle size distribution for high efficiency, low pressure drop and best total economy in chromatographic purifications.

In figures 1 and 2, the SEM photographs of Kromasil 3.5 µm and 10 µm are shown.

Fig. 1 - Kromasil 3.5 µm

Fig. 2 - Kromasil 10 µm

Batch to batch reproducibility

The manufacturing of Kromasil spherical silica is done according to the well-known sol-gel technique which gives optimized chemical and mechanical stability, pore structure and reproducible surface area.

Development, production and marketing of the Kromasil packings are ISO 9001 certified.

Extensive control and testing is performed through the whole production process, ensuring the highest possible reproducibility. Proof of this can be seen in figures 3 and 4, showing the reproducibility of batches of Kromasil.
Kromasil packings are manufactured in batches over 100 kg, in a modern plant with capacity of several tonnes per year.

Fig. 3 - Batch to batch reproducibility of 50 batches of Kromasil 100 Å, C18 with respect to α and k'.
Conditions:
Eluent: acetonitrile/water (70/30)
Flow rate: 2 ml/min.
Detection: UV 254 nm
Column size: 4.6 × 250 mm

Fig. 4 - Batch to batch reproducibility of 50 batches of Kromasil 100 Å, C18 with respect to BET and ligand density.
Conditions:
Eluent: acetonitrile/water (70/30)
Flow rate: 2 ml/min.
Detection: UV 254 nm
Column size: 4.6 × 250 mm