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Kromasil Eternity – Applications
Purification of crude amitriptyline
Semi-prep purification of crude amitriptyline on a short semi-prep column.
| Stationary Phase: | Kromasil Eternity-5-C18 |
| Column size: | 21.2 × 50 mm |
| Mobile Phase: | acetonitrile/25 mM NH4HCO3 pH 10.5 |
| Gradient: | 45-80% acetonitrile/10 min |
| Flow rate: | 21 ml/min |
| Temperature: | 25°C |
| Detection: | UV 254 nm |
| Stationary Phase: | Kromasil Eternity-2.5-C18 |
| Column size: | 4.6 × 50 mm |
| Mobile Phase: | acetonitrile/10 mM NH4HCO3 pH 10.5 (55/45) |
| Flow rate: | 1.7 ml/min |
| Temperature: | 25°C |
| Detection: | UV 254 nm |
Determination of β-carotene content in three carrot varieties
Analytical determination of β-carotene extracted from grinded carrots by acetone extraction. A yellow, an orange and a purple carrot were analyzed.
| Stationary Phase: | Kromasil Eternity-2.5-C18 |
| Column size: | 4.6 × 100 mm |
| Mobile Phase: | acetonitrile/2-propanol/water (70/25/5) |
| Flow rate: | 1.7 ml/min |
| Temperature: | 25°C |
| Detection: | UV/VIS 470 nm |
Substance
- 1 = β-carotene
More details about this application note
More Eternity applications in the Application Guide
Here is a list of applications run on Kromasil Eternity to be found in the application guide:
- Total hits: 13
- β-blockers Separation of selected β1 receptor selective antagonists on Kromasil Eternity C18
- Anestesics Separation of sodium channel blockers / alkaloids on Kromasil eternity C18
- Antihistamines Separation of antihistaminic coumpounds on Kromasil Eternity C18
- Aromatics Separation of aromatic coumpounds on Kromasil Eternity PhenylHexyl
- Benzoic acid and derivatives Separation of benzoic acid and derivatives on Kromasil Eternity C18
- Caffeine and metabolites Separation of caffeines and its methabolites on Kromasil Eternity C18
- Nucleobases Separation of nucleobases on Kromasil Eternity PhenylHexyl
- Peptides Separation of various peptides on Kromasil Eternity C18
- Pesticides Separation of herbicides in methanol on Kromasil Eternity C18
- Pesticides Separation of some photosynthesis inhibiting herbicides of the urea family on Kromasil Eternity C18 (UHPLC)
- Polycyclic aromatic hydrocarbons Separation of some basic polycyclic aromatic hydrocarbons on Kromasil Eternity C18
- Separation of basic APIs at high pH Various basic substances are separated at pH 12 on Kromasil Eternity C18
- Sulfa drugs Separation of sulfa drugs in methanol on Kromasil Eternity C18
