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Caffeine and metabolites

Separation of caffeines and its metabolites on Kromasil ClassicShell C18

  • 1 = theobromine
    s00235
  • 2 = theophylline
    s00234
  • 3 = caffeine
    s00233
chromatogram for g1047
Column:Kromasil ClassicShell-2.5-C18 2.1 × 50 mm
Part number:NH2CLD05
Eluent:acetonitrile / water / 0.1% formic acid
Gradient:0 min: 3%, 4 min, 30% acetonitrile
Flow rate:0.5 ml/min
Temperature:ambient
Detection:UV @ 254 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.1
ClassicShellC182.5 NH2CLD__