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Newsletter September 2011 from Kromasil |
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KromaNews:Prep & Production |
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Recent changes: the former and the new General ManagerDuring the last one and a half years Separation Products has undergone some important changes, as Domingo Sanchez, one of the founders of the group, has retired as General Manager.
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Basic methodology for method development in preparative HPLCQuite often one meets people with excellent knowledge and experience in analytical HPLC, and while much theory and underlying equations can be used on a preparative scale, a lot may differ. The main difference is the scope of the separation. In analytical chromatography you are most certainly looking for information in some way, either identification or quantification (or both) of one or several peaks in one chromatogram. However, in preparative chromatography the isolation of a single substance at a certain purity is usually the main goal. This difference has a large impact on how to develop the chromatographic method in order to optimize factors such as yield, purity and loadability. ![]()
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Gradient or isocratic elution in preparative reversed phase HPLCA common question that often arises in the discussion between prep chromatographers is when and why one should choose either isocratic or gradient elution? Since we in the Kromasil group have a long experience of developing large scale separations we would like to share some of these experiences with the readers of KromaNews. ![]()
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