Newsletter March 2014 from Kromasil

KromaNews:Prep & Production

A brief history of preparative liquid chromatography

Traditional preparative liquid chromatography was born in 1903 when the Russian botanist M. Twsett made the first separation of carotene pigments. The columns he used were made of glass, they were packed with gravels and were basically operated at atmospheric pressure. This situation kept unchanged for many years, until the 1960’s when organic chemists began to use stainless steel columns operated at a few bars.

How did DAC columns change preparative chromatography?

Improved loadability for basic APIs
- run at high pH

The main proportion of all synthetic pharmaceutical APIs are basic in nature, and will in many cases exhibit an increased loadability, and hence productivity, at high pH. Basic peptides, oligos, PNAs, etc, will also benefit from high pH separation methods.

Running at the right pH may open new opportunities