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Kromasil® UHPLC columns with 1.8 µm particles are the latest technology advancement from Nouryon that enable your laboratory to achieve unmatched resolution, decreased turnaround time and minimized laboratory costs.

Kromasil® chromatography columns now affords your laboratory with a unique opportunity as these new UHPLC columns provide the scalability and reliability solution you have been searching for. With the solid Kromasil® brand, you can now use the same quality products as your counterparts across your organization, including production. Best of all, you can now transfer UHPLC methods to HPLC and your colleagues will be able to scale them up to semi-prep for small quantity purification and subsequent manufacturing of pharmaceuticals and other industrial applications, when required. You can achieve this seamlessly with the same Kromasil® quality stationary phase, with reproducibility second to none, now in smaller particle sizes and corresponding column configurations.

QC test

chromatogram of QC test, columns and box
Chromatogram, columns and box
  • Conditions
  • Column:Kromasil 100-1.8-C18 2.1 × 50 mm
  • Sample:1 = dimethyl phthalate
  •  2 = toluene
  •  3 = biphenyl
  •  4 = phenanthrene
  • Mobile phase: acetonitrile/water (65/35)
  • Flow rate:0.6 ml/min
  • Temperature:35 °C
  • Detection:UV @ 254 nm

Maintaining selectivity

  • Conditions
  • Columns:Kromasil 100-dp-C4 4.6 x 50 mm
  •  for dp from 2.5 to 5 µm
  •  Kromasil 100-1.8-C4 2.1 x 50 mm
  • Mobile phase:acetonitrile
  • Sample:vitamin D & E
  • Flow rates:5.0 µm: 1.0 ml/min
  •  3.5 µm: 1.5 ml/min
  •  2.5 µm: 2.0 ml/min
  •  1.8 µm: 0.6 ml/min
  • Temperature:20 °C
  • Detection:UV @ 215 nm
chromatograms at 4 particle sizes
Same selectivity in the fraction of the time.

Applications

Antibiotics

chromatogram of separation of 3 antibiotic compounds
  • Conditions
  • Column:Kromasil 100-1.8-C18 2.1 × 50 mm
  • Sample:1 = ciprofloxacin
  •  2 = levofloxacin
  •  3 = moxifloxacin
  • Mobile phase:acetonitrile / potassium phosphate, 20 mM, pH 2.5 / triethylamine (20/80/0.2)
  • Flow rate:0.6 ml/min
  • Temperature:35 °C
  • Detection:UV @ 280 nm

Anti-inflammatory drugs

chromatogram of separation of 6 anti-inflamatory substances
  • Conditions
  • Column:Kromasil 100-1.8-C18 2.1 × 50 mm
  • Sample:1 = piroxicam
  •  2 = ketorolac
  •  3 = ketoprofen
  •  4 = diflunisal
  •  5 = diclofenac
  •  6 = ibuprofen
  • Mobile phase:acetonitrile/water + 0.1% TFA
  • Gradient:acetonitrile; 0 min: 50%, 2 min: 95%
  • Flow rate:0.5 ml/min
  • Temperature:45 °C
  • Detection:UV @ 254 nm

Tryptic digest of Cytochrome C

chromatogram of tryptic digest of Cytochrom C
  • Conditions
  • Column:Kromasil 100-1.8-C18 2.1 × 50 mm
  • Mobile phase:acetonitrile/water + 0.1% TFA
  • Gradient:acetonitrile; 0 min: 5%, 60 min: 50%
  • Flow rate:0.3 ml/min
  • Temperature:35 °C
  • Detection:UV @ 210 nm, with baseline subtraction

Availability

Availability of Kromasil® 1.8 µm columns

Kromasil® 1.8 µm is based on the 100 Å product line, and is available with C4, C8 and C18 derivatizations, in 2.1 to 4.6 mm I.D. columns.

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.0
100 ÅC41.8 MF1CSD__ MF1CSC__
100 ÅC81.8 MF1CMD__ MF1CMC__
100 ÅC181.8 MF1CLD__ MF1CLC__

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