With our 25 years of experience in the silica-based field, AkzoNobel presents to you one of the strongest and most successful chromatographic products in the market to date, now in 2.5 µm particle size. You can use Kromasil columns with 2.5 µm particles in both HPLC and UHPLC systems.

This flexibility affords you the opportunity to develop and adapt methods for fast turnaround under HPLC conditions or to develop robust UHPLC methods. These Kromasil columns with 2.5 µm particles are based on very narrow specification ranges, resulting in chromatographic columns with excellent resolution, and the well known Kromasil column-to-column reproducibility.


Addressing sustainability

chromatogram with 6 peaks
QC-test on Kromasil 2.5 μm
  • Conditions
  • Column: Kromasil 100-2.5-C18 4.6 × 50 mm
  • Sample: 1 = sodium nitrite
  •   2 = benzamide
  •   3 = methyl benzoate
  •   4 = toluene
  •   5 = propyl benzene
  •   6 = butyl benzene
  • Mobile phase: acetonitrile/water (70/30)
  • Flow rate: 2.0 ml/min
  • Temperature: 25 °C
  • Detection: UV @ 229 nm

As these new Kromasil columns contain the same stationary phase as other larger particle size Kromasil columns, your existing Kromasil HPLC methods can now be easily scaled to 2.5 µm particle columns. This straightforward, fast scale-down approach supports the latest laboratory trends worldwide whether your organizations strive to move faster to market, manage the laboratory more effectively, or achieve sustainability goals from a solvent consumption perspective.

The strong Kromasil brand, which spans across all particle sizes, allows an easier transfer of methods developed on 2.5 µm particles to other departments, such as quality control. They can also be a good start for the scale up and purification of small, medium and large quantities of compounds.

You can feel secure using this new 2.5 µm platform from Kromasil, a brand used and trusted by scientists worldwide. The new product line is designed for users with flexibility in mind, who may be required to run under UHPLC or HPLC conditions.


Scalability

From the company that began by supplying silica-based material for the purification of insulin, with consistent quality in state-of-the-art facilities, Kromasil is now proud to offer its customers a complete range of particle sizes with excellent selectivity, column-to-column reproducibility, and peak shape.

chromatograms of separation at 3 particle sizes
Separation of vitamins on 2.5, 3.5 and 5 µm particles
  • Common conditions
  • Column size: 4.6 × 50 mm
  • Sample:vitamin D & E
  • Mobile phase: acetonitrile
  • Flow rate: 1.0 ml/min
  • Temperature: 20 °C
  • Detection: UV @ 215 nm

Applications

Mixture of 15 pharmaceutical compounds

  • Conditions
  • Column: Kromasil 100-2.5-C18 4.6 × 100 mm
  • Sample: 1 = pyridine
  •   2 = acetaminophen
  •   3 = pindolol
  •   4 = acebutolol
  •   5 = sulfathiazole
  •   6 = chloropheniramine
  •   7 = triprolidine
  •   8 = nortriptyline
  •   9 = benzyl alcohol
  •   10 = prednisolone
  •   11= phenol
  •   12 = 4-nitrobenzoic acid
  •   13 = 2-hydroxy-5-methylbenzoic acid
  •   14 = diflunisal
  •   15 = hexanophenon
  • Mobile phase: acetonitrile/water + 0.1% formic acid
  • Gradient: acetonitrile; 0 min: 2%, 0.2 min: 2 %
  •   4.7 min: 98%, 5.7 min: 98%, 5.7 min: 2%
  • Flow rate: 1.5 ml/min
  • Temperature: 20 °C
  • Detection: UV @ 254 nm

β-blockers

  • Conditions
  • Column: Kromasil 100-2.5-C18 4.6 × 50 mm
  • Sample: 1 = sotalol
  •   2 = nadolol
  •   3 = timolol
  •   4 = metoprolol
  •   5 = alprenolol
  • Mobile phase:acetonitrile/water + 0.1% TFA
  • Gradient:acetonitrile; 0 min: 5%, 2.7 min: 70%
  • Flow rate:3.0 ml/min
  • Temperature:50 °C
  • Detection:UV @ 230 nm

Availability

Availability of Kromasil 2.5 µm columns

Kromasil 2.5 µm is based on the 100 Å product line, and is available with C4, C8 and C18 derivatizations, in 2.1 to 4.6 mm I.D. columns. Select a column length in the table for more details about the actual product product.

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.04.6
100 ÅC42.5 MH2CSD__ MH2CSC__ MH2CSA__
100 ÅC82.5 MH2CMD__ MH2CMC__ MH2CMA__
100 ÅC182.5 MH2CLD__ MH2CLC__ MH2CLA__

Kromasil Eternity

Kromasil Chiral

Kromasil SFC

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