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The Kromasil® diC4 stationary phase is a HPLC phase targeting the purification of peptide and biomolecules. With 100 Å pore size it has shown to be highly efficient to reach purity levels over 99.5% for GLP-1 receptor agonists like Liraglutide and Semaglutide, even with crudes with a target product purity as low as 15%. In 300 Å, the purification of larger biomolecules can also be addressed.
Kromasil® diC4 has strong C4 characteristics in terms of capacity and hydrophobicity. The main difference is a steric selectivity in between a C18 and a C4, more like a C8.
Better loadability on first step gives diC4 a huge lead over C4 in the second step where high purity must be achieved.
Kromasil® diC4, a totally porous spherical silica with a dibutyl silane derivatization, is available in bulk with 100 or 300 Å pore size and 10 to 16 μm particle size. These materials are also available in prepacked columns:
Particle size, [μm] | ||||
---|---|---|---|---|
Family | Phase | 10 | 13 | 16 |
100 Å | diC4 | M10DCblk | M13DCblk | |
300 Å | diC4 | L10DCblk | L16DCblk |
Column diameters, [mm] | ||||||||
---|---|---|---|---|---|---|---|---|
Family | Phase | dp [μm] | 4.0 | 4.6 | 10 | 20 | 30 | 50 |
100 Å | diC4 | 10 | M10DCB__ | M10DCA__ | M10DCP__ | M10DCO__ | M10DCR__ | M10DCT__ |
100 Å | diC4 | 13 | M13DCB__ | M13DCA__ | M13DCP__ | M13DCO__ | M13DCR__ | M13DCT__ |
300 Å | diC4 | 10 | L10DCB__ | L10DCA__ | L10DCP__ | L10DCO__ | ||
300 Å | diC4 | 16 | L16DCB__ | L16DCA__ | L16DCP__ | L16DCO__ | L16DCR__ | L16DCT__ |