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Kromasil® EternityShell columns are a further expansion of the Kromasil® analytical portfolio. Kromasil® EternityShell columns have been designed for users who expect to perform separations between pH 1 and 12 and look for fast separations using solid-core technology.

Kromasil® EternityShell columns

The Kromasil® EternityShell columns are based on solid-core particles and used in the analysis of pharmaceutical, clinical, environmental, food and beverage and industrial samples.

These new columns are packed with 2.5 μm solid-core particles. Kromasil® EternityShell are aimed for fast separations similarly to the already existent Kromasil® EternityXT columns with 1.8 and 2.5 μm particles.

Illustration of a splitted solid-core particle

Extended pH range

The Kromasil® EternityShell C18 columns are robust and stable beyond traditional silica columns. Offering excellent pH flexibility between pH 1 and 12, These EternityShell columns are for users that need to work under an almost full pH range, including acidic conditions but also at tougher high pH for the analysis for bases. The new chromatographic material is endcapped, has a surface area of 110 m2/g and a carbon coverage of 8%, providing a first rate alternative selectivity for reversed phase chromatography.

pH range
Work with confidence in a pH range between 1 and 12.

More choices for analysis

Users can now choose from both fully porous and solid-core Kromasil® small particle columns to achieve their results. Here the user exploits differences in retention according to the stationary phase characteristics with maintained selectivity. Considering Kromasil® EternityShell and EternityXT, with a lower ratio of porous material available for the first one, the interaction between the compounds and the stationary phase is lower, resulting in a quicker elution. When considering Kromasil® ClassicShell and EternityShell chromatographic results, the characteristics of each stationary phase combined with the given substances allows the user to tune in the retention time.

Separation of Steroids on 3 C18 phases
In this separation of steroids, the same analysis is carried out on three C18 columns from different Kromasil® families.
  • Conditions
  • Columns: Kromasil EternityShell-2.5-C18 2.1 x 50 mm
  •   Kromasil ClassicShell-2.5-C18 2.1 x 50 mm
  •   Kromasil EternityXT-2.5-C18 2.1 x 50 mm
  • Mobile phase: acetonitrile / water
  • Gradient: 0 min: 35%, 1.5 min: 60% acetonitrile
  • Substances: 1 = cortisone, 2 = corticosterone, 3 = deoxycorticosterone
  • Flow rate: 0.6 ml/min
  • Temperature: ambient
  • Detection: UV @ 254 nm

Applications

Fast separations under harsh conditions

Separation of local anesthetics
Separation of local anesthetics.
  • Conditions
  • Column:Kromasil EternityShell-2.5-C18 2.1 x 50 mmm
  • Mobile phase:acetonitrile / water, 10 mM ammonium carbonate, pH 10.5
  • Gradient: 0 min: 30%, 1.5 min: 90% acetonitrile
  • Flow rate:0.7 ml/min
  • Temperature:ambient
  • Detection:UV @ 220 nm
  • Sample:1 = benzocaine, 2 = lidocaine,
  •  3 = tetracaine, 4 = bupivacaine
Separation of tricyclic drugs
Separation of tricyclic drugs.
  • Conditions
  • Column:Kromasil EternityShell-2.5-C18 2.1 x 50 mmm
  • Mobile phase:acetonitrile / water + 0.1% DEA
  • Gradient: 0 min: 60%, 1.5 min: 80% acetonitrile
  • Flow rate:0.6 ml/min
  • Temperature:ambient
  • Detection:UV @ 254 nm
  • Sample:1 = carbamazepine, 2 = nortriptyline,
  •  3 = imipramine, 4 = amitriptyline
Separation of aromatics
Separation of aromatics
  • Conditions
  • Column:Kromasil EternityShell-2.5-C18 2.1 x 50 mmm
  • Mobile phase:acetonitrile / water
  • Gradient: 0 min: 60%, 2 min: 85% acetonitrile
  • Flow rate:0.6 ml/min
  • Temperature:ambient
  • Detection:UV @ 254 nm
  • Sample:1 = toluene, 2 = naphthalene, 3 = biphenyl,
  •   4 = acenaphthene, 5 = phenanthrene,
  •   6 = anthracene, 7 = o-terphenyl
  •   8 = trihenylene

Availability

Availability of Kromasil® EternityShell columns

Kromasil® EternityShell is based on 2.5 μm solid-core particles, and is available with C18 derivatization, in 2.1 mm I.D. HPLC columns with lenght 50 mm and 100 mm.

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