Traditional silica-based reversed phase materials very often have an upper limit for use at neutral to slightly basic pH. At higher pH levels, the silica matrix starts to dissolve. With Kromasil Classic RP phases this limit has been moved up to pH 9.5, and in some cases, even higher. With the Eternity platform, the boundaries are moved beyond what could be expected from the strongest silica matrix.
Up to pH 12
The first generation of Eternity C18 set a new standard for column lifetime expectations for hybrid materials. With EternityXT C18, users get the flexibility to develop methods for quick UHPLC analysis as well as isolation and large-scale purification between pH 1-12, for long-term use.
Long-term chemical stability
Flexibility at your fingertips
The main proportion of all synthetic pharmaceutical APIs are basic in nature, and will exhibit an increased loadability, and hence productivity, at a high pH. Basic peptides, oligos and PNAs will also benefit from high pH separation methods. in addition, it is possible to sanitize or regenerate Kromasil EternityXT in-column (CIP) even using 1 M NaOH when necessary. 1 m NaOH is a standard in biochromatography for polymeric resins.
With Kromasil EternityXT, users have the flexibility to develop analytical and separation methods for virtually the entire pH range, and to sanitize or regenerate the column using conditions previously reserved only for polymeric resins. This gives scientists the best of both worlds: highest performance and excellent stability at high pH.