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Frequently Asked Questions

Which detector flow cell should I use for my column?

In order to get the best possible sensitivity, it is important to work with the correct flow cell / column combination. Standard HPLC systems are optimized for 4.0 to 4.6 mm ID columns. If smaller ID columns are used, the standard detector flow cell will likely contribute to the extra column volume to such an extent that peak efficiency is compromised. The table below indicates combinations of detector flow cells and column dimensions:

Column ID [mm] Typical flow rate [mL/min] Flow cell volume [μL]
4.6 1.0 13-15
4.0 0.75 13-15
3.0 0.5 5-9
2.1 0.2 5-9
1.0 0.05 1-2