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Certain buffers or organic solvents interfere with UV detection. Only mobile phases with a UV cut-off below the detection wavelength will not compromise the signal sensitivity.
The tables below show the UV cut-off of the most commonly used buffers, solvents and additives in HPLC.
| pH value | Buffer | UV cut-off [nm] |
|---|---|---|
| 2.0 – 3.0 | Phosphate | 210 |
| 3.5 – 5.5 | Acetate | 240 |
| 4.0 – 6.0 | Citrate | 250 |
| 6.0 – 8.5 | Phosphate | 210 |
| 7.0 – 9.0 | TRIS | 225 |
| 8.0 – 10.5 | Borate | 210 |
| Solvent | UV cut-off [nm] |
|---|---|
| Acetone | 330 |
| Acetonitrile | 190 |
| Dichloromethane | 233 |
| Dimethoxy sulfoxide | 268 |
| Dioxan | 215 |
| Ethanol | 210 |
| Ethylacetate | 256 |
| Hexane | 195 |
| Heptane | 200 |
| Methanol | 205 |
| Methyl-ethyl ketone | 329 |
| Methyl t-butyl ether | 210 |
| N,N-Dimethylformamide | 268 |
| Propan-2-ol (IPA) | 205 |
| Tetrahydrofuran | 210 |
| Toluene | 284 |
| Additive | UV cut-off [nm] |
|---|---|
| Acetic acid | 230 |
| Diethylamine | 210 |
| Formic acid | 210 |
| Triethylamine | 235 |
| Trifluoroacetic acid | 210 |