Frequently Asked Questions

Which mobile phases work with my detection wavelength?

Certain buffers or organic solvents interfere with UV detection. Only mobile phases with a UV cut-off below the detection wavelength will not compromise the signal sensitivity.

The tables below show the UV cut-off of the most commonly used buffers, solvents and additives in HPLC.

pH value Buffer UV cut-off [nm]
2.0 – 3.0 Phosphate 210
3.5 – 5.5 Acetate 240
4.0 – 6.0 Citrate 250
6.0 – 8.5 Phosphate 210
7.0 – 9.0 TRIS 225
8.0 – 10.5 Borate 210
Solvent UV cut-off [nm]
Acetone 330
Acetonitrile 190
Dichloromethane 233
Dimethoxy sulfoxide 268
Dioxan 215
Ethanol 210
Ethylacetate 256
Hexane 195
Heptane 200
Methanol 205
Methyl-ethyl ketone 329
Methyl t-butyl ether 210
N,N-Dimethylformamide 268
Propan-2-ol (IPA) 205
Tetrahydrofuran 210
Toluene 284
Additive UV cut-off [nm]
Acetic acid 230
Diethylamine 210
Formic acid 210
Triethylamine 235
Trifluoroacetic acid 210
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