Frequently Asked Questions

Which are common buffers used in HPLC?


pH buffers
Salt pKA Buffer
pH range
equilibrium UV
cut-off
Ammonium acetate 4.76
9.2
3.8-5.8
8.2-10.2
HAc ↔ Ac
NH4+ ↔ NH3
205
Ammonium formate 3.8
9.2
2.8-4.8
8.2-10.2
HCOOH ↔ HCOO
NH4+ ↔ NH3
200
Potassium phosphate 2.1
7.2
1.1-3.1
6.2-8.2
H3PO4 ↔ H2PO4
H2PO4 ↔ HPO42−
190
Triethylammonium acetate 4.76
11.01
3.8-5.8
10.-12.0
HAc ↔ Ac
Et3NH + ↔ Et3N
235
Additives
Salt pKA Buffer
pH range
equilibrium UV
cut-off
Acetic acid 4.76 - HAc ↔ Ac 205
Formic acid 3.8 - HCOOH ↔ HCOO 200
Trifluroacetic acid 0.3 - F3CCOOH ↔ F3CCOO 210
Trietylamine 11.01 - Et3NH+ ↔ Et3N 235
Diethylamine 10.5 - Et2NH2+ ↔ Et2HN 235
Ammonium hydroxide 9.2 - NH4+ ↔ NH3 190
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