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The performance is best checked by re-running the separation shown on the enclosed test chromatogram. The exact conditions are mentioned. The sample amount should not exceed the following quantities:
| Column Ø
[mm ID] |
test sample
[µg] |
| 2.1 | 2 |
| 4.6 | 10 |
| 10 | 50 |
| 21.2 | 225 |
In order to achieve representative plate counts, it is important to reduce the extra column volumes, e.g. correct tubing has to be installed between injection and the column and from the column to the detector.