Peak tailing can occur due to numerous reasons. The problem can be identified according to the following scheme:
For larger ID columns (>10 mm) radial temperature gradient can also cause peak tailing. In order to avoid such, it is recommended to use a column oven. The column should be 1-2°C warmer than the mobile phase in order to compensate for the friction heat generated in the core of the column.
If neither of the above applies, the tailing can also be caused by irregularities in the column packing process or by a partially clogged inlet frit leading to an inhomogeneous flow profile.
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