Frequently Asked Questions

Can a Kromasil AmyCoat or CelluCoat chiral column be used in both Normal Phase and Reversed Phase mode?

Yes, the columns can be operated under normal phase, polar mode and reversed phase conditions. The regular columns are packed to give highest possible column efficiency under normal phase and polar mode conditions (alcohol or acetonitrile, no alkane). They can be converted to reversed phase via 100% ethanol or isopropanol as an intermediate solvent. Of course, the columns can also be re-conditioned to the original mode. By converting the column, one will observe a loss in column efficiency when comparing the plate count in RP vs. NP mode. For regular use under RP conditions, we recommend the purchase of columns that are packed giving highest possible efficiency under reversed phase conditions, AmyCoat RP and CelluCoat RP.


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