Javascript message

This site uses javascript. If javascript is disabled in your browser, some functionality may be impaired.

Frequently Asked Questions

Can the column be operated with 100% aqueous mobile phase?

The reversed phase Kromasil columns C4, C8 and C18 should be operated with at least 5% organic modifier. Under 100% aqueous conditions, the columns are likely to undergo dewetting which means the mobile phase will be expelled from the porous system due to surface tension. In this case the analytes elute more or less with the void volume. The problem arises mainly if the flow is stopped. If a column has been dewetted, it can easily be restored by pumping mobile phase with at least 50% organic modifier through the column for ca 40 min.

Kromasil C18(w) is specialised phase variant that is fully compatible with 100% aqueous conditions. Find out more about it on the product page.