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Frequently Asked Questions

What does HILIC mean?

HILIC means Hydrophilic Interaction Liquid chromatography and is a technique used for separating highly polar substances. It can also be described as aqueous normal phase chromatography. The stationary phase has to be of polar character, e.g. silica, diol, amine, amide or zwitterionic. As a mobile phase, generally acetonitrile / water mixtures are used, where water is the strong component, thus exhibiting a higher elution strength than acetonitrile. Thus, HILIC is a sub-category of normal phase chromatography and shows opposite elution pattern to reversed phase.