This site uses javascript. If javascript is disabled in your browser, some functionality may be impaired.
For each particle size, there is an optimal linear flow rate with respect to column efficiency, according to the van Deemter plot.
| 3.5 µm | 5 µm | 10 µm | 13 µm | 16 µm | |
| Linear flow rate
(cm/min) |
7.1 | 4.7 | 3.3 | 2.4 | 1.9 |
| 2.1 mm ID | 0.3 | 0.2 | - | - | - |
| 4.6 mm ID | 1.5 | 1.0 | 0.7 | 0.5 | 0.4 |
| 10 mm ID | - | 4.7 | 3.7 | 2.4 | 1.9 |
| 21.2 mm ID | - | 21 | 15 | 10 | 8.5 |
For shorter separation times, the flow rate can however be increased until the pressure drop limit of the HPLC pump is reached, e.g. ca 400 bar.