For each particle size, there is an optimal linear flow rate with respect to column efficiency, according to the van Deemter plot.
|3.5 µm||5 µm||10 µm||13 µm||16 µm|
|Linear flow rate
|2.1 mm ID||0.3||0.2||-||-||-|
|4.6 mm ID||1.5||1.0||0.7||0.5||0.4|
|10 mm ID||-||4.7||3.7||2.4||1.9|
|21.2 mm ID||-||21||15||10||8.5|
For shorter separation times, the flow rate can however be increased until the pressure drop limit of the HPLC pump is reached, e.g. ca 400 bar.
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