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Peptides

Separation of 9 peptides

  • 1 = oxytocin
    s00225
  • 2 = bradykinin
    s00226
  • 3 = methionine enkephalin
    s00227
  • 4 = angiotensin II
    s00230
  • 5 = leucine enkephalin
    s00228
  • 6 = angiotensin I
    s00229
  • 7 = insulin
    s00231
  • 8 = lysozyme
    s00400
  • 9 = melittin
    s00232
chromatogram for g0025
Column:Kromasil 100-5-C8 4.6 × 250 mm
Part number:M05CMA25
Eluent A:acetonitrile/water/TFA (10/90/0.1)
Eluent B:acetonitrile/water/TFA (90/10/0.1)
Gradient:0 min 0% B, 8 min 25% B, 20 min 75% B
Flow rate:2 ml/min
Temperature:ambient
Detection:UV 254 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.03.94.04.610203050
100 ÅC85 M05CMD__ M05CMC__ M05CMJ__ M05CMB__ M05CMA__ M05CMP__ M05CMO__ M05CMV__ M05CMW__