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Caffeine and metabolites

Separation of caffeines and its metabolites on Kromasil Eternity C18

  • 1 = xanthine
    s00046
  • 2 = theobromine
    s00235
  • 3 = theophylline
    s00234
  • 4 = caffeine
    s00233
chromatogram for g1015
Column:Kromasil Eternity 5-C18 4.6 × 250 mm
Part number:E05CLA25
Eluent:methanol/water (20/80)
Flow rate:1.5 ml/min
Temperature:30°C
Detection:UV 273 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.04.04.6102030
EternityC185 E05CLD__ E05CLC__ E05CLB__ E05CLA__ E05CLP__ E05CLO__ E05CLV__