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Separation of basic APIs at high pH

Various basic substances are separated at pH 12 on Kromasil Eternity C18

  • 1 = salsolinol
    s00236
  • 2 = benzylamine
    s00237
  • 3 = propafenone
    s00238
  • 4 = verapamil
    s00239
  • 5 = butaclamol
    s00653
chromatogram for g1019
Column:Kromasil Eternity 2.5-C18 4.6 × 100 mm
Part number:EH2CLA10
Eluent:methanol/water, sodium phosphate buffer pH 12
Gradient:0 - 2 min: 5%, 2.1 min: 60%, 15 min: 80% (methanol)
Flow rate:1.0 ml/min
Temperature:35°C
Detection:UV 254 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.04.6
EternityC182.5 EH2CLD__ EH2CLC__ EH2CLA__