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Nucleobases

Separation of nucleobases on Kromasil Eternity PhenylHexyl

  • 1 = cytosine
    s00654
  • 2 = uracil
    s00188
  • 3 = thymine
    s00655
  • 4 = adenine
    s00656
chromatogram for g1020
Column:Kromasil Eternity 2.5-PhenylHexyl 4.6 × 50 mm
Part number:EH2PXA05
Eluent:potassium phosphate buffer, 25 mM, pH 7.85
Flow rate:1.2 ml/min
Temperature:30°C
Detection:UV 210 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.04.6
EternityPhenylHexyl2.5 EH2PXD__ EH2PXC__ EH2PXA__