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Nucleobases

Separation of nucleobases under HILIC conditions

  • 1 = thymine
    s00655
  • 2 = uracil
    s00188
  • 3 = adenine
    s00656
  • 4 = guanine
    s00659
  • 5 = cytosine
    s00654
chromatogram for g1023
Column:Kromasil 60-5-HILIC-D 4.6 × 150 mm
Part number:S05HDA15
Eluent:acetonitrile/ammonium acetate buffer, 100 mM, pH 6.3 (85/15)
Flow rate:2 ml/min
Temperature:ambient
Detection:UV @ 254 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.04.04.610203050
60 ÅHILIC-D5 S05HDD__ S05HDC__ S05HDB__ S05HDA__ S05HDP__ S05HDO__ S05HDV__ S05HDW__