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Vitamins

Separation of water soluble vitamins in HILIC mode

  • 1 = nicotinamide
    s00389
  • 2 = riboflavine
    s00391
  • 3 = pyroxidine
    s00660
  • 4 = p-aminobenzoic acid
    s00336
  • 5 = nicotinic acid
    s00661
  • 6 = L-ascorbic acid
    s00388
  • 7 = thiamine chloride
    s00392
  • 8 = cobalamin
    s00662
  • 9 = folic acid
    s00663
chromatogram for g1024
Column:Kromasil 60-5-HILIC-D 4.6 × 150 mm
Part number:S05HDA15
Eluent:acetonitrile/ammonium acetate buffer, 100 mM, pH 6.3
Gradient:0 min: 75%, 10 min: 55% acetonitrile
Flow rate:1.0 ml/min
Temperature:ambient
Detection:UV @ 254 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.04.04.610203050
60 ÅHILIC-D5 S05HDD__ S05HDC__ S05HDB__ S05HDA__ S05HDP__ S05HDO__ S05HDV__ S05HDW__