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Steroids

Separation of steroids by SFC on Kromasil SFC SIL

  • 1 = deoxycorticosterone
    s00667
  • 2 = cortisone
    s00668
  • 3 = corticosterone
    s00669
  • 4 = hydrocortisone
    s00670
chromatogram for g1029
Column:Kromasil SFC-2.5-SIL 3.0 × 150 mm
Part number:FH2SIC15
Eluent:CO2 / methanol
Gradient:0 min: 5%, 10 min: 30% methanol
Temperature:40∞C
Flow rate:2 ml/min
Outlet pressure:120 bar
Detection:ES-MS and UV @ 220 nm

Reference

AstraZeneca application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]3.04.6
SFCSIL2.5 FH2SIC__ FH2SIA__