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Antibiotics

Separation of sulfa drugs by SFC on Kromasil SFC Diol

  • 1 = sulfadimethoxine
    s00114
  • 2 = sulfamethoxypyridazine
    s00112
  • 3 = sulfaquinoxaline
    s00110
  • 4 = sulfadiazine
    s00672
  • 5 = sulfathiazole
    s00189
chromatogram for g1036
Column:Kromasil SFC-2.5-Diol 3.0 × 150 mm
Part number:FH2DIC15
Eluent:CO2 / methanol
Gradient:0 min: 5%, 10 min: 30% methanol
Temperature:40°C
Flow rate:2 ml/min
Outlet pressure:120 bar
Detection:ES-MS and UV @ 220 nm

Reference

AstraZeneca application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]3.04.6
SFCDiol2.5 FH2DIC__ FH2DIA__