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β-blockers

Separation of β-blockers by SFC on Kromasil SFC cyano

  • 1 = alprenolol
    s00626
  • 2 = propanolol
    s00637
  • 3 = acebutolol
    s00673
  • 4 = pindolol
    s00651
chromatogram for g1042
Column:Kromasil SFC-2.5-CN 3.0 × 150 mm
Part number:FH2CNC15
Eluent:CO2 / methanol + 20 mM ammonia
Gradient:0 min: 5%, 10 min: 30% methanol
Temperature:40°C
Flow rate:2 ml/min
Outlet pressure:120 bar
Detection:ES-MS and UV @ 220 nm

Reference

AstraZeneca application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]3.04.6
SFCCN2.5 FH2CNC__ FH2CNA__