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Caffeine and metabolites

Separation of caffeines and its metabolites on Kromasil EternityXT PhenylHexyl

  • 1 = theobromine
    s00235
  • 2 = paraxanthine
    s00516
  • 3 = theophylline
    s00234
  • 4 = caffeine
    s00233
chromatogram for g1098
Column:Kromasil EternityXT-5-PhenylHexyl 4.6 × 250 mm
Part number:X05PXA25
Eluent:methanol / water / formic acid [40/60/0.1]
Flow rate:1.0 ml/min
Temperature:30 °C
Detection:UV 254 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.14.6102021.230
EternityXTPhenylHexyl5 X05PXD__ X05PXA__ X05PXP__ X05PXO__ X05PXQ__ X05PXV__