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Separation of cannabinoids

Identification of 5 cannabinoids on Kromasil C18

  • 1 = cannabidiolic acid
    s00900
  • 2 = cannabidiol
    s00901
  • 3 = cannabinol
    s00902
  • 4 = δ9-tetrahydrocannabinol
    s00903
  • 5 = tetrahydrocannabinolic acid
    s00904
chromatogram for g1201
Column:Kromasil 100-5-C18 4.6 × 150 mm
Part number:M05CLA15
Eluent:acetonitrile/water/0.1% formic acid
Gradient:0 min: 72.5%; 0.5 min: 78%; 1.5 min: 85%; 3 min: 90%; 4.5 min: 92.5%; 5.5 min: 85%; 6.5 min: 72.5%, acetonitrile
Flow rate:1.5 ml/min
Temperature:50°C
Detection:UV @ 271 nm

Reference

Courtesy of Longboard Scientific Consulting in San Diego, CA, USA

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.03.94.04.610203050
100 ÅC185 M05CLD__ M05CLC__ M05CLJ__ M05CLB__ M05CLA__ M05CLP__ M05CLO__ M05CLV__ M05CLW__