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Separation of short oligonucleotides

Separation of deoxythymidylate-based oligonucleotides on Kromasil C18

  • 1 = dT5
    s00905
  • 2 = dT10
    s00906
  • 3 = dT15
    s00907
  • 4 = dT20
    s00908
chromatogram for g1202
Column:Kromasil 100-2.5-C18 3.0 × 150 mm
Part number:MH2CLC15
Eluent:acetonitrile / water + 50 mM triethylammonium acetate
Gradient:0 min: 10.15%; 84 min: 21.99% acetonitrile
Flow rate:0.3 ml/min
Temperature:50 °C
Detection:UV @ 260 nm

Reference

Courtesy of Dr Martin Enmark, Karlstad University, Sweden. Work supported by the Swedish Knowledge Foundation for the KKS SYNERGY project 2016 "BIO-QC: Quality Control and Purification for New Biological Drugs" (grant number 20170059)

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.04.6
100 ÅC182.5 MH2CLD__ MH2CLC__ MH2CLA__