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Separation of short oligonucleotides

Separation of deoxythymidylate-based oligonucleotides on Kromasil C4

  • 1 = dT5
  • 2 = dT10
  • 3 = dT15
  • 4 = dT20
chromatogram for g1203
Column:Kromasil 100-2.5-C4 3.0 × 150 mm
Part number:MH2CSC15
Eluent:acetonitrile / water + 5 mM tributylammonium acetate
Gradient:0 min: 36%; 60 min: 60% acetonitrile
Flow rate:0.42 ml/min
Temperature:50 °C
Detection:UV @ 260 nm


Courtesy of Dr Martin Enmark, Karlstad University, Sweden. Work supported by the Swedish Knowledge Foundation for the KKS SYNERGY project 2016 "BIO-QC: Quality Control and Purification for New Biological Drugs" (grant number 20170059)

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]
100 ÅC42.5 MH2CSD__ MH2CSC__ MH2CSA__