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Separation of short oligonucleotides

Separation of deoxythymidylate-based oligonucleotides on Kromasil Phenyl

  • 1 = dTs5
    s00909
  • 2 = dTs10
    s00910
  • 3 = dTs15
    s00911
  • 4 = dTs20
    s00912
chromatogram for g1204
Column:Kromasil 100-2.5-Phenyl 3 × 150 mm
Part number: (non-standard product/column size)
Eluent:acetonitrile / water + 5 mM tributylammonium acetate
Gradient:0 min: 36%; 60 min: 60% acetonitrile
Flow rate:0.42 ml/min
Temperature:50 °C
Detection:UV @ 260 nm

Reference

Courtesy of Dr Martin Enmark, Karlstad University, Sweden. Work supported by the Swedish Knowledge Foundation for the KKS SYNERGY project 2016 "BIO-QC: Quality Control and Purification for New Biological Drugs" (grant number 20170059)

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]2.13.03.94.04.610203050
100 ÅPhenyl5 M05PHD__ M05PHC__ M05PHJ__ M05PHB__ M05PHA__ M05PHP__ M05PHO__ M05PHV__ M05PHW__
100 ÅPhenyl10 M10PHJ__ M10PHB__ M10PHA__ M10PHP__ M10PHO__ M10PHR__ M10PHT__
100 ÅPhenyl16 M16PHB__ M16PHA__ M16PHP__ M16PHO__ M16PHR__ M16PHT__