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Separation of deoxythymidylate-based oligonucleotides on Kromasil Phenyl
Column: | Kromasil 100-2.5-Phenyl 3 × 150 mm |
Part number: | (non-standard product/column size) |
Eluent: | acetonitrile / water + 5 mM tributylammonium acetate |
Gradient: | 0 min: 36%; 60 min: 60% acetonitrile |
Flow rate: | 0.42 ml/min |
Temperature: | 50 °C |
Detection: | UV @ 260 nm |
Courtesy of Dr Martin Enmark, Karlstad University, Sweden. Work supported by the Swedish Knowledge Foundation for the KKS SYNERGY project 2016 "BIO-QC: Quality Control and Purification for New Biological Drugs" (grant number 20170059)
Column diameters, [mm] | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Family | Phase | dp [μm] | 2.1 | 3.0 | 3.9 | 4.0 | 4.6 | 10 | 20 | 30 | 50 |
100 Å | Phenyl | 5 | M05PHD__ | M05PHC__ | M05PHJ__ | M05PHB__ | M05PHA__ | M05PHP__ | M05PHO__ | M05PHV__ | M05PHW__ |
100 Å | Phenyl | 10 | M10PHJ__ | M10PHB__ | M10PHA__ | M10PHP__ | M10PHO__ | M10PHR__ | M10PHT__ | ||
100 Å | Phenyl | 16 | M16PHB__ | M16PHA__ | M16PHP__ | M16PHO__ | M16PHR__ | M16PHT__ |