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Serotonin and metabolites

Separation of serotonin and metabolites on Kromasil C18(w)

  • 1 = 5-hydroxy-L-tryptophan
    s00920
  • 2 = serotonin
    s00921
  • 3 = L-tryptophan
    s00922
  • 4 = 5-hydroxyindoleacetic acid
    s00923
chromatogram for g1212
Column:Kromasil 100-5-C18(w) 4.6 × 150 mm
Part number:M05WLA15
Eluent:acetonitrile / ammonium acetate, 50 mM, pH 4 (10/90)
Flow rate:1 ml/min
Temperature:ambient
Detection:UV @ 280 nm

Reference

Nouryon application note

Kromasil columns available with the stationary phase used in this application

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]4.04.6102030
100 ÅC18(w)5 M05WLB__ M05WLA__ M05WLP__ M05WLO__ M05WLV__