Kromasil SFC columns for analysis and isolation
Based on 100 Å pore size, 2.5 and 5 μm particles, Kromasil SFC columns give users the opportunity of fast separations. The columns are tailor-made for research, discovery and routine analysis.
Many options
Kromasil SFC columns are the answer for fast separations. These columns are now being delivered in cyano, diol, silica, 2-ethylpyridine and fused organo-silane chemistries for the laboratory scientist to separate a wide range of substances, from non-polar to strongly polar compounds.
The stationary phase quintet
Separation of β‑blockers
By using this standard set of Kromasil SFC columns, the user can efficiently screen the material that works best for a given sample.
- Conditions
- Packing:Kromasil SFC, 2.5 µm
- Phases:SIL ,
CN ,
Diol ,
2EP and
XT
- Column size:3.0 x 150 mm
- Eluent:CO2 / methanol + 20 mM ammonia
- Gradient:0 min: 5%, 10 min: 30% methanol
- Outlet pressure:120 bar
-
- Substances:1 = alprenolol
- 2 = propranolol
- 3 = acebutolol
- 4 = pindolol
- Temperature:40°C
- Detection:ES-MS and UV @ 220 nm
Fast separations
Medium and high-throughput laboratories working with green technology and seeking to improve turnaround time are now able to take advantage of the separation power of the new Kromasil SFC 2.5 μm family of columns. With the chromatographic power of Kromasil SFC phases available in short and narrow columns, users can easily achieve baseline resolution and ultra-fast separations.
- Conditions
- Column:Kromasil SFC-2.5-2EP 3.0 × 50 mm
- Eluent:CO2 / methanol
- Gradient:0 min: 10%, 1 min: 20% methanol
- Flow rate:2.5 ml/min
- Temperature:40°C
- Outlet pressure:120 bar
-
- Detection:ES-MS and UV @ 220 nm
- Substances:1 = deoxycorticosterone
- 2 = corticosterone
- 3 = cortisone
- 4 = hydrocortisone
Orthogonality between phases
In general, the 2EP functionality is known for its benefits in terms of selectivity and retention in SFC, and it has the specific benefits towards the separation of basic compounds, where peak shape is significantly improved. Therefore the 2EP chemistry is seen as the workhorse for SFC.
With the introduction of Kromasil SFC XT we can now offer a SFC phase that greatly compliments 2EP showing orthogonality towards 2EP and other common SFC phases when run under standard SFC conditions. This new material provides additional tools to SFC users around the world.
Elution order for common acidic, neutral and basic substances on selected SFC phases. Relative elution time increases downwards in the figure.
Alternative selectivity
The new Kromasil SFC XT offers additional interactions with the stationary phase surface, creating alternative selectivities.
Separation of anilines on Kromasil SFC SIL and XT
- Conditions
- Columns:Kromasil SFC-2.5-SIL 3.0 × 150 mm
- Kromasil SFC-2.5-XT 3.0 × 150 mm
- Eluent:CO2 / methanol
- Gradient:0 min: 2%, 7 min: 9% methanol
- Temperature:40°C
- Outlet pressure:110 bar
-
- Detection:UV @ 220 nm
- Substances:1 = aniline
- 2 = ethylaniline
- 3 = 2-nitroaniline
- 4 = 3-nitroaniline
- 4 = 4-nitroaniline
Easy scale up and purififcation
All Kromasil SFC phases are available as 2.5 and 5 μm particles sizes with columns up to 30 mm i.d. In this way we can offer products that give users the possibility to seamlessly transition between different particle and column sizes and easily scale up their separations.
Similarly to LC, almost all preparative separation starts at analytical scale using either smaller particles, more narrow columns or a combination of the two. By developing the preparative method on an analytical scale, SFC users are able to minimize solvent and sample usage while shorten time for method development.
Maintained selectivity
Whether in analytical or preparative scale, 2.5 or 5 μm particles, Kromasil offers the same material produced in a similar way with similar specifications. This means users can be sure that the separation itself will stay identical during scale up.
- Conditions
- Column:Kromasil SFC-2.5-XT 3.0 × 100 mm
- Eluent:CO2 / 20% methanol
- Flow rate:1.5 ml/min
- Temperature:40 °C
- Outlet pressure:110 bar
- Detection:UV @ 254 nm
- Substances:1 = phenol
- 2 = phenytoin
- 3 = hydrocortisone
- Conditions
- Column:Kromasil SFC-5-XT 10 × 250 mm
- Eluent:CO2 / 20% methanol
- Flow rate:8.3 ml/min
- Temperature:40 °C
- Outlet pressure:100 bar
- Detection:UV @ 254 nm
- Substances:1 = phenol
- 2 = phenytoin
- 3 = hydrocortisone
Great loadability
Because of the high surface availability of our SFC line, our materials show great loadability which makes them excellent choices for preparative purification under overloaded conditions.
Purification of prednisolone
- Conditions
- Column:Kromasil SFC-2.5-XT 3.0 × 100 mm
- Eluent:CO2 / methanol
- Gradient:0 min: 10%, 3 min: 20% methanol
- Flow rate:2.0 ml/min
- Temperature:40 °C
- Outlet pressure:120 bar
- Detection:UV @ 230 nm
- Conditions
- Column:Kromasil SFC-5-XT 10 × 250 mm
- Eluent:CO2 / 20% methanol
- Flow rate:8.0 ml/min
- Temperature:40 °C
- Outlet pressure:100 bar
- Detection:UV @ 254 nm
- Loading:16 mg
Applications
Find here a couple of applications illustrating the use of all Kromasil SFC phases. For other applications and variants on phases not displayed below, please consult the Application Guide.
Local anaesthetics
- Conditions
- Columns:Kromasil SFC-2.5-SIL 3.0 × 150 mm
- Kromasil SFC-2.5-CN 3.0 × 150 mm
- Kromasil SFC-2.5-2EP 3.0 × 150 mm
- Eluent:CO2 / methanol + 20 mM ammonia
- Gradient:0 min: 5%, 5 min: 30% methanol
- Flow rate:2.0 ml/min
- Temperature:40°C
-
- Outlet pressure:110 bar
- Detection:UV @ 230 nm
- Substances:1 = lidocaine
- 2 = benzocaine
- 3 = bupivacaine
- 4 = tetracaine
- 5 = procaine
Hydrobenzenes
- Conditions
- Columns:Kromasil SFC-2.5-SIL 3.0 × 150 mm
- Kromasil SFC-2.5-Diol 3.0 × 150 mm
- Kromasil SFC-2.5-XT 3.0 × 150 mm
- Eluent:CO2 / methanol
- Gradient:0 min: 2%, 10 min: 25% methanol
- Flow rate:2.0 ml/min
- Temperature:40°C
-
- Outlet pressure:110 bar
- Detection:UV @ 220 nm
- Substances:1 = benzyl alcohol
- 2 = resorcinol
- 3 = catechol
- 4 = hydroquinone
- 5 = phloroglucinol
Product characteristics
Kromasil SFC is based on first-in-class, perfectly spherical, porous Kromasil silica particles with 100 Å pore size and particle sizes of 2.5 and 5 μm. Furthermore, Kromasil SFC XT is the result of a patented organic/inorganic merged silica technology.
Availability
The Kromasil® SFC columns are available in 2.5 and 5 μm particle size with the following surface chemistries:
- silica (SIL)
- cyano (CN)
- diol (Diol)
- 2-ethylpiridine (2EP)
- fused organo-silane (XT)
KIT is a 4 column kit for screening studies with one column of each phases of the following phases in one box: SIL, CN, Diol and 2EP.
Availability of Kromasil® SFC columns