Kromasil SFC

Based on 100 Å pore size, 2.5 and 5 μm particles, Kromasil SFC columns give users the opportunity of fast separations. The columns are tailor-made for research, discovery and routine analysis.

Many options

Kromasil SFC columns are the answer for fast separations. These columns are now being delivered in cyano, diol, silica, 2-ethylpyridine and fused organo-silane chemistries for the laboratory scientist to separate a wide range of substances, from non-polar to strongly polar compounds.

The stationary phase quintet

Separation of β‑blockers

Fast separations

Medium and high-throughput laboratories working with green technology and seeking to improve turnaround time are now able to take advantage of the separation power of the new Kromasil SFC 2.5 μm family of columns. With the chromatographic power of Kromasil SFC phases available in short and narrow columns, users can easily achieve baseline resolution and ultra-fast separations.

Separation of steroids

Orthogonality between phases

In general, the 2EP functionality is known for its benefits in terms of selectivity and retention in SFC, and it has the specific benefits towards the separation of basic compounds, where peak shape is significantly improved. Therefore the 2EP chemistry is seen as the workhorse for SFC.

With the introduction of Kromasil SFC XT we can now offer a SFC phase that greatly compliments 2EP showing orthogonality towards 2EP and other common SFC phases when run under standard SFC conditions. This new material provides additional tools to SFC users around the world.

Alternative selectivity

The new Kromasil SFC XT offers additional interactions with the stationary phase surface, creating alternative selectivities.

Easy scale up and purififcation

All Kromasil SFC phases are available as 2.5 and 5 μm particles sizes with columns up to 30 mm i.d. In this way we can offer products that give users the possibility to seamlessly transition between different particle and column sizes and easily scale up their separations.

Similarly to LC, almost all preparative separation starts at analytical scale using either smaller particles, more narrow columns or a combination of the two. By developing the preparative method on an analytical scale, SFC users are able to minimize solvent and sample usage while shorten time for method development.

Maintained selectivity

Whether in analytical or preparative scale, 2.5 or 5 μm particles, Kromasil offers the same material produced in a similar way with similar specifications. This means users can be sure that the separation itself will stay identical during scale up.

• Conditions
• Column:Kromasil SFC-2.5-XT 3.0 × 100 mm
• Eluent:CO₂ / 20% methanol
• Flow rate:1.5 ml/min
• Temperature:40 °C
• Outlet pressure:110 bar
• Detection:UV @ 254 nm
• Substances:1 = phenol
• 2 = phenytoin
• 3 = hydrocortisone

• Conditions
• Column:Kromasil SFC-5-XT 10 × 250 mm
• Eluent:CO₂ / 20% methanol
• Flow rate:8.3 ml/min
• Temperature:40 °C
• Outlet pressure:100 bar
• Detection:UV @ 254 nm
• Substances:1 = phenol
• 2 = phenytoin
• 3 = hydrocortisone

Great loadability

Because of the high surface availability of our SFC line, our materials show great loadability which makes them excellent choices for preparative purification under overloaded conditions.

• Conditions
• Column:Kromasil SFC-2.5-XT 3.0 × 100 mm
• Eluent:CO₂ / methanol
• Gradient:0 min: 10%, 3 min: 20% methanol
• Flow rate:2.0 ml/min
• Temperature:40 °C
• Outlet pressure:120 bar
• Detection:UV @ 230 nm

• Conditions
• Column:Kromasil SFC-5-XT 10 × 250 mm
• Eluent:CO₂ / 20% methanol
• Flow rate:8.0 ml/min
• Temperature:40 °C
• Outlet pressure:100 bar
• Detection:UV @ 254 nm
• Loading:16 mg

Applications

Find here a couple of applications illustrating the use of all Kromasil SFC phases. For other applications and variants on phases not displayed below, please consult the Application Guide.

Local anaesthetics

Hydrobenzenes

Product characteristics

Kromasil SFC is based on first-in-class, perfectly spherical, porous Kromasil silica particles with 100 Å pore size and particle sizes of 2.5 and 5 μm. Furthermore, Kromasil SFC XT is the result of a patented organic/inorganic merged silica technology.

Availability

The Kromasil^® SFC columns are available in 2.5 and 5 μm particle size with the following surface chemistries:

• silica (SIL)
• cyano (CN)
• diol (Diol)
• 2-ethylpiridine (2EP)
• fused organo-silane (XT)

KIT is a 4 column kit for screening studies with one column of each phases of the following phases in one box: SIL, CN, Diol and 2EP.