Kromasil SFC columns for analysis and isolation

Based on 100 Å pore size, 2.5 and 5 μm particles, Kromasil SFC columns give users the opportunity of fast separations. The columns are tailor-made for research, discovery and routine analysis.

Many options

Kromasil SFC columns are the answer for fast separations. These columns are now being delivered in cyano, diol, silica, 2-ethylpyridine and fused organo-silane chemistries for the laboratory scientist to separate a wide range of substances, from non-polar to strongly polar compounds.

The stationary phase quintet

Separation of β‑blockers

By using this standard set of Kromasil SFC columns, the user can efficiently screen the material that works best for a given sample.
Separation of beta-blockers on Kromasil SFC SIL
Separation of beta-blockers on Kromasil SFC CN
Separation of beta-blockers on Kromasil SFC Diol
Separation of beta-blockers on Kromasil SFC 2EP
Separation of beta-blockers on Kromasil SFC XT
  • Conditions
  • Packing:Kromasil SFC, 2.5 µm
  • Phases:SIL , CN , Diol , 2EP and XT
  • Column size:3.0 x 150 mm
  • Eluent:CO2 / methanol + 20 mM ammonia
  • Gradient:0 min: 5%, 10 min: 30% methanol
  • Outlet pressure:120 bar
  • Substances:1 = alprenolol
  • 2 = propranolol
  • 3 = acebutolol
  • 4 = pindolol
  • Temperature:40°C
  • Detection:ES-MS and UV @ 220 nm

Fast separations

Medium and high-throughput laboratories working with green technology and seeking to improve turnaround time are now able to take advantage of the separation power of the new Kromasil SFC 2.5 μm family of columns. With the chromatographic power of Kromasil SFC phases available in short and narrow columns, users can easily achieve baseline resolution and ultra-fast separations.


Separation of steroids on Kromasil SFC 2EP

Separation of steroids

  • Conditions
  • Column:Kromasil SFC-2.5-2EP 3.0 × 50 mm
  • Eluent:CO2 / methanol
  • Gradient:0 min: 10%, 1 min: 20% methanol
  • Flow rate:2.5 ml/min
  • Temperature:40°C
  • Outlet pressure:120 bar
  • Detection:ES-MS and UV @ 220 nm
  • Substances:1 = deoxycorticosterone
  • 2 = corticosterone
  • 3 = cortisone
  • 4 = hydrocortisone

Orthogonality between phases

In general, the 2EP functionality is known for its bene ts in terms of selectivity and retention in SFC, and it has the speci c bene ts towards the separation of basic compounds, where peak shape is signi cantly improved. Therefore the 2EP chemistry is seen as the workhorse for SFC.

With the introduction of Kromasil SFC XT we can now offer a SFC phase that greatly compliments 2EP showing orthogonality towards 2EP and other common SFC phases when run under standard SFC conditions. This new material provides additional tools to SFC users around the world.

Elution order for common acidic, neutral and basic substances on selected SFC phases. Relative elution time increases downwards in the figure.
Elution order of 32 substances over 3 selected SFC phases

Alternative selectivity

The new Kromasil SFC XT offers additional interactions with the stationary phase surface, creating alternative selectivities.

Separation of anilines on Kromasil SFC SIL and XT
Separation of anlilines drugs
 on Kromasil SFC SIL
Separation of anlilines drugs
 on Kromasil SFC XT
  • Conditions
  • Columns:Kromasil SFC-2.5-SIL 3.0 × 150 mm
  • Kromasil SFC-2.5-XT 3.0 × 150 mm
  • Eluent:CO2 / methanol
  • Gradient:0 min: 2%, 7 min: 9% methanol
  • Temperature:40°C
  • Outlet pressure:110 bar
  • Detection:UV @ 220 nm
  • Substances:1 = aniline
  • 2 = ethylaniline
  • 3 = 2-nitroaniline
  • 4 = 3-nitroaniline
  • 4 = 4-nitroaniline

Easy scale up and purififcation

All Kromasil SFC phases are available as 2.5 and 5 μm particles sizes with columns up to 30 mm i.d. In this way we can offer products that give users the possibility to seamlessly transition between different particle and column sizes and easily scale up their separations.

Similarly to LC, almost all preparative separation starts at analytical scale using either smaller particles, more narrow columns or a combination of the two. By developing the preparative method on an analytical scale, SFC users are able to minimize solvent and sample usage while shorten time for method development.

Maintained selectivity

Whether in analytical or preparative scale, 2.5 or 5 μm particles, Kromasil offers the same material produced in a similar way with similar specifications. This means users can be sure that the separation itself will stay identical during scale up.

chromatogram of analytical prep-mix injection
  • Conditions
  • Column:Kromasil SFC-2.5-XT 3.0 × 100 mm
  • Eluent:CO2 / 20% methanol
  • Flow rate:1.5 ml/min
  • Temperature:40 °C
  • Outlet pressure:110 bar
  • Detection:UV @ 254 nm
  • Substances:1 = phenol
  • 2 = phenytoin
  • 3 = hydrocortisone
chromatogram of semi.prep prep-mix injection
  • Conditions
  • Column:Kromasil SFC-5-XT 10 × 250 mm
  • Eluent:CO2 / 20% methanol
  • Flow rate:8.3 ml/min
  • Temperature:40 °C
  • Outlet pressure:100 bar
  • Detection:UV @ 254 nm
  • Substances:1 = phenol
  • 2 = phenytoin
  • 3 = hydrocortisone

Great loadability

Because of the high surface availability of our SFC line, our materials show great loadability which makes them excellent choices for preparative purification under overloaded conditions.

Purification of prednisolone
chromatogram of analytical prep-mix injection
  • Conditions
  • Column:Kromasil SFC-2.5-XT 3.0 × 100 mm
  • Eluent:CO2 / methanol
  • Gradient:0 min: 10%, 3 min: 20% methanol
  • Flow rate:2.0 ml/min
  • Temperature:40 °C
  • Outlet pressure:120 bar
  • Detection:UV @ 230 nm
chromatogram of semi.prep prednisolone injection
  • Conditions
  • Column:Kromasil SFC-5-XT 10 × 250 mm
  • Eluent:CO2 / 20% methanol
  • Flow rate:8.0 ml/min
  • Temperature:40 °C
  • Outlet pressure:100 bar
  • Detection:UV @ 254 nm
  • Loading:16 mg

Applications

Find here a couple of applications illustrating the use of all Kromasil SFC phases. For other applications and variants on phases not displayed below, please consult the Application Guide.

Local anaesthetics

chromatogram of local anesthetics separation on Kromasil SFC SIL
chromatogram of local anesthetics separation on Kromasil SFC CN
chromatogram of local anesthetics separation on Kromasil SFC 2EP
  • Conditions
  • Columns:Kromasil SFC-2.5-SIL 3.0 × 150 mm
  • Kromasil SFC-2.5-CN 3.0 × 150 mm
  • Kromasil SFC-2.5-2EP 3.0 × 150 mm
  • Eluent:CO2 / methanol + 20 mM ammonia
  • Gradient:0 min: 5%, 5 min: 30% methanol
  • Flow rate:2.0 ml/min
  • Temperature:40°C
  • Outlet pressure:110 bar
  • Detection:UV @ 230 nm
  • Substances:1 = lidocaine
  • 2 = benzocaine
  • 3 = bupivacaine
  • 4 = tetracaine
  • 5 = procaine

Hydrobenzenes

chromatogram of hydrobenzenes separation on Kromasil SFC SIL
chromatogram of hydrobenzenes separation on Kromasil SFC Diol
chromatogram of hydrobenzeness separation on Kromasil SFC XT
  • Conditions
  • Columns:Kromasil SFC-2.5-SIL 3.0 × 150 mm
  • Kromasil SFC-2.5-Diol 3.0 × 150 mm
  • Kromasil SFC-2.5-XT 3.0 × 150 mm
  • Eluent:CO2 / methanol
  • Gradient:0 min: 2%, 10 min: 25% methanol
  • Flow rate:2.0 ml/min
  • Temperature:40°C
  • Outlet pressure:110 bar
  • Detection:UV @ 220 nm
  • Substances:1 = benzyl alcohol
  • 2 = resorcinol
  • 3 = catechol
  • 4 = hydroquinone
  • 5 = phloroglucinol

Product characteristics

Kromasil SFC is based on first-in-class, perfectly spherical, porous Kromasil silica particles with 100 Å pore size and particle sizes of 2.5 and 5 μm. Furthermore, Kromasil SFC XT is the result of a patented organic/inorganic merged silica technology.

schematic structure of Kromasil SFC SIL
schematic structure of Kromasil SFC Diol
schematic structure of Kromasil SFC XT
schematic structure of Kromasil SFC CN
schematic structure of Kromasil SFC 2EP

Availability

The Kromasil SFC columns are available in 2.5 and 5 μm particle size with the following surface chemistries:

  • silica (SIL)
  • cyano (CN)
  • diol (Diol)
  • 2-ethylpiridine (2EP)
  • fused organo-silane (XT)

KIT is a 4 column kit for screening studies with one column of each phases of the following phases in one box: SIL, CN, Diol and 2EP.

Availability of Kromasil SFC columns

Click/touch the dot for the combination of stationary phase and column diameter of your interest to access column length availability and further options.
Column diameters, [mm]
FamilyPhasedp [μm]3.04.61021.230
SFCSIL2.5 FH2SIC__ FH2SIA__
SFCSIL5 F05SIC__ F05SIA__ F05SIP__ F05SIQ__ F05SIR__
SFCDiol2.5 FH2DIC__ FH2DIA__
SFCDiol5 F05DIC__ F05DIA__ F05DIP__ F05DIQ__ F05DIR__
SFCCN2.5 FH2CNC__ FH2CNA__
SFCCN5 F05CNC__ F05CNA__ F05CNP__ F05CNQ__ F05CNR__
SFC2EP2.5 FH2EPC__ FH2EPA__
SFC2EP5 F05EPC__ F05EPA__ F05EPP__ F05EPQ__ F05EPR__
SFCXT2.5 FH2XTC__ FH2XTA__
SFCXT5 F05XTC__ F05XTA__ F05XTP__ F05XTQ__ F05XTR__
SFCKIT2.5 FH2FKC__
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