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GLP-1 agonist purification toolbox
- Methodology to maximize purity and throughput in Liraglutide and Semaglutide purificationGLP-1 agonists are a family of peptides that have become popular in the early treatment of diabetes type ll.
A set of purification methods have been developed using Kromasil® stationary phases, including 100 Å diC4, for removal of impurities in peptide crudes. -
Kromasil® diC4 stationary phase for peptide and biomolecule separations
With the new 100 Å diC4, unlock:
- Increased yield
- Reduced use of organic solvent
- Higher loadability
- Higher productivity -
Thriving in water - Fully wettable phase for analytical and prep HPLC
The Kromasil wettable phase is the latest innovation of Nouryon, an HPLC phase that has been specifically designed for polar compounds. This Kromasil 100 Å C18(w) permits loading and run start in 100% aqueous, enabling new opportunities for engineers and scientists working with analytical or prep HPLC.
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Kromasil SFC - Introducing Kromasil SFC XT
Designed for green technology - SFC columns for analysis and isolation.
Based on 100 Å pore size, 2.5 and 5 μm particles, Kromasil SFC columns give users the opportunity of fast separations. The columns are tailor-made for research, discovery and routine analysis. -
Kromasil EternityXT - UHPLC/HPLC columns with extended lifetime
The family of Kromasil EternityXT UHPLC and HPLC columns is now also available with a C8 phase for extended separation alternatives.
More information about EternityXT columns on this website or in the Kromasil Guide. -
Kromasil EternityXT prep family - long-life stationary phases for prep HPLC
Kromasil EternityXT is a family of unparalleled stationary phases that keep you in the forefront of purification technology. The Kromasil EternityXT bulk materials support you in your preparative chromatography needs even under extreme harsh conditions, like cleaning-in-place, where the mechanical and chemical stability of the packing could be challenged.
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Kromasil EternityShell - Columns with solid-core particles for harsh conditions
More options — with a wider range of pH conditions
Kromasil EternityShell columns are a further expansion of the Kromasil analytical portfolio. Kromasil EternityShell columns have been designed for users who expect to perform separations between pH 1 and 12 and look for fast separations using solid-core technology. -
Kromasil ClassicShell - Expanding opportunities
More choices in your analytical tool box.
Kromasil ClassicShell family of columns is an expansion of the Kromasil analytical columns portfolio to provide scientists with more options for the analysis of laboratory samples. -
Kromasil guide
Second edition
This is the anthology of the world of chromatography with Kromasil.
• Presentation of all product platforms: Classic, Eternity, Chiral and SFC
• Comprehensive availability of stationary phases and columns
• Aspects of chromatography
• Application guide with examples from our labs and the litterature
View online -
Kromasil prep - Boost your productivity, lower your cost
Kromasil’s business idea since its introduction in 1988 is to offer you the lowest possible total separation cost by providing the highest quality silica-based HPLC, SMB and SFC packing material available
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Product data sheets – Kromasil 60 Å
SIL, CN, Diol and HILIC-D
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Product data sheets – Kromasil 100 Å
SIL, C4, C8, C18, NH2 and Phenyl
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Product data sheets – Kromasil 300 Å
SIL, C4, diC4, C8 and C18
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Product data sheets - Kromasil EternityXT
C8 and C18
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Product data sheets – Kromasil Chiral
Characteristics and compatible mobile phases for AmyCoat and CelluCoat
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Purification of GLP-1 agonists using a novel diC4 silica-based stationary phase
André C. Garcia, Therése Tran and Fredrik Limé (2023)
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Advantage of using chemically and mechanically stable reversed chromatography phase
Fredrik Limé (2023)
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Chromatographic toolbox for large scale purification of GLP-1 agonists
Fredrik Lime, Therése Tran, Börje Persson and Joakim Högblom (2022)
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Wettable C18 stationary phase for loading and analysis using 100% aqueous mobile phase
Fredrik Limé & Peter Gidlund (2022)
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Chromatographic toolbox for purification of GLP-1 agonists
Fredrik Lime, Therése Tran, Börje Persson & Joakim Högblom (2022)
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Kromasil wettable phase for successful separations in aqueous phase
Fredik Limé, Börje Person, Jagoda Serafin, Cecilia Mazza and Marija Berzius-Zarins (2019)
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Investigation of impurity profiles of GLP-1 analogue Liraglutide
Therése Tran, Joakim Högblom and Fredrik Limé (2019)
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Analytical versus Preparative and Production-Scale HPLC: Some Considerations
Robert Fredriksson, Innovations in Pharmaceutical Technology, Issue 40 (2012), 16-18